NF1 Rabbit Polyclonal Antibody

SKU: orb632946

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Description

Rabbit polyclonal antibody to Neurofibromin

Research Area

Cancer, Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	WB: 1:500 - 1:1000; IHC: 1:50 - 1:500; IF: 1:50 - 1:500
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Neurofibromin
Target	NF1
Molecular Weight	319 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Neurofibromin, neurofibromin 1, NF1, Neurofibromatosis-related protein NF-1

Phospho-YB1 (Ser102) Rabbit Polyclonal Antibody [orb5632]

Applications:	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Guinea pig, Porcine
Reactivity:	Human, Mouse, Rabbit, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 7
NFIC/CTF Rabbit Polyclonal Antibody [orb654354]
Applications:
ELISA, FC, IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 4
NFIB Rabbit Polyclonal Antibody [orb157973]
Applications:
FC, WB
Predicted Reactivity:
Bovine, Gallus, Porcine, Rabbit, Rat, Sheep
Reactivity:
Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl

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Hela cells were subjected to SDS PAGE followed by western blot with orb632946 (NF1 Antibody) at dilution of 1:600.

Immunofluorescent analysis of HeLa cells using orb632946 (NF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb632946 (NF1 Antibody) at dilution of 1:200.

Quick Database Links

Gene Symbol

NF1

UniProt

P21359

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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