YB1 Rabbit Polyclonal Antibody

SKU: orb100303

Description

YB1 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human YB1 (51-150/324aa)
• Target: YBX1
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1; 1700102N10Rik; EF1A; MSY1; mYB-1a; Byb1; Cbfa; YBOX1_HUMAN; YBX1; CCAAT-binding transcription factor I subunit A (CBF-A); DNA-binding protein B (DBPB); Enhancer factor I subunit A (EFI-A); Nuclease-sensitive element-binding protein 1; Y-box transcription factor; YBOX1_MOUSE; Msy-1; YBOX1_RAT;

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YB1) Polyclonal Antibody, Unconjugated (orb100303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YB1) Polyclonal Antibody, Unconjugated (orb100303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HL-60 cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-YB1 (orb100303) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kDa, Observed band size: 50 kDa.

Tissue/cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-YBX-1/YB1 Polyclonal Antibody, Unconjugated (orb100303) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

YBX1

UniProt

P67809

Łukasz A Poniatowski et al. Detection of Y-box binding protein 1 (YB-1) in serum and cerebrospinal fluid with its changed brain expression as a possible supplemental diagnostic tool for traumatic brain injury Forensic Sci Int, (2026)

PubMed: 42019275

Applications

IHC

Reactivity

Human