neuronal pentraxin 1 Antibody

SKU: orb1248346

Description

neuronal pentraxin 1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour. Additional bands of unknown identity was also consistently observed at 38 and 14kDa. These bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ERVKIETALTSLHQR
• Target: neuronal pentraxin 1
• Molecular Weight: Approx 50kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NPTX1, neuronal pentraxin I, NP1, NP-I, neuronal pentraxin-1

orb1248346 (1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248346 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

neuronal pentraxin 1

UniProt

Q15818

RefSeq (Protein):NP_002513.2