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Item 1 of 7
Item 1 of 7
NPTX1-Y344 Antibody
Catalog Number: orb1929405
| Catalog Number | orb1929405 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This NPTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from human NPTX1. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q15818 |
| MW | 47122 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25 |
| Antibody Type | Primary Antibody |
| Clone Number | RB15369 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Neuronal pentraxin-1, NP1, Neuronal pentraxin I, N Read more... |
| Note | For research use only |
| NCBI | NP_002513.2 |
Staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Western blot analysis of NPTX1-Y344 in mouse brain tissue lysates (35 ug/lane).NPTX1 (arrow) was detected using the purified Pab.
All lanes: Anti-NPTX1Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NPTX1-Y344 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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