Neuroligin 1 Antibody

• Catalog Number: orb150874
• Category: Antibodies
• Description: Mouse Anti-Rat Neuroligin 1 Monoclonal IgG1
• Target: Neuroligin 1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) > 40% identity with Neuroligin-2 and -3.
• UniProt ID: Q62765
• MW: 120kDa
• Tested applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: 2 µg/ml of SMC-463 was sufficient for detection of Neuroligin-1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N97A/31 (Formerly sold as S97A-31)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NLG 1 Antibody, KIAA1070 Antibody, MGC45115 Antibo
• Research Area: Disease Biomarkers, Neuroscience
• Note: For research use only
• Entrez: 116647
• NCBI: NP_446320.1
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone N97A/31. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1 Antibody (D) Composite.