Neuroligin 1 Antibody

• Catalog Number: orb19055
• Category: Antibodies
• Description: Goat polyclonal antibody to NLGN1
• Target: Neuroligin 1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TAKQDDPKQQ
• RRID: AB_10749103
• MW: 92
• Tested applications: ELISA, IF
• Dilution range: ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NLGN1 antibody, anti KIAA1070 antibody, anti
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 22871
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of U251 cells using NLGN1 antibody

Immunofluorescence analysis of U2OS cells using NLGN1 antibody