Neuroligin 1 Antibody

SKU: orb19055

Description

Goat polyclonal antibody to NLGN1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Neuroligin 1
• Protein Sequence: TAKQDDPKQQ
• Molecular Weight: 92
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NLGN1 antibody, anti KIAA1070 antibody, anti MGC45115 antibody, anti neuroligin 1 antibody, anti OTTHUMP00000211275 antibody, anti OTTHUMP00000211276 antibody

Immunofluorescence analysis of U251 cells using NLGN1 antibody

Immunofluorescence analysis of U2OS cells using NLGN1 antibody

Quick Database Links

Gene Symbol

Neuroligin 1

NCBI Gene ID

22871