NEFH Antibody

SKU: orb1252726

Description

NEFH Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, WB
• Reactivity: Gallus, Human, Mouse, Porcine, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (2)Prediluted format : incubate for 30 min at RT (3)The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.1. The NF421 antibody is specific to the heavy subunit.2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NF421
• Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)
• Target: NEFH
• Purification: Protein G purified Neurofilament antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

Human cerebellum stained with Neurofilament antibody (NF421).

SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NF421). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

NEFH

UniProt

P12036