Neurofilament NF-H Antibody

SKU: orb319585

Description

Our Anti-Neurofilament NF-H primary antibody from Biorbyt is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1:50000, IHC: 1:5000-1:25,000, ICC: 1:25,000
• Reactivity: Gallus, Mammal

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Purified bovine NF-H
• Target: Neurofilament NF-H
• Molecular Weight: 200
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 30 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody

Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (Cat. orb319588, 1:5000, red) and anti-Neurofilament-H (Cat. orb319585, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (Cat. orb319585, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (Cat. orb319549, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.

Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (Cat. orb319585, green, 1:25,000). The section is colabeled with anti-calbindin (Cat. orb378472 , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.

Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (Cat. orb319704, 1:500, red) and anti-Neurofilament H (Cat. orb319585, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (Cat. orb319585, red, 1:25,000) and anti-NFL antibody (Cat. orb319586, green, 1:100).

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.

Quick Database Links

Gene Symbol

Neurofilament NF-H

UniProt

P12036