Neurofilament Antibody Cocktail (Heavy + Light)

• Catalog Number: orb749970
• Category: Antibodies
• Description: This mAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Triton-X 100 insoluble proteins of rat brain (RT-97) and Neurofilaments from porcine spinal cord (NR-4) were used as the immunogen for the Neurofilament antibody cocktail.
• UniProt ID: P12036
• Tested applications: FACS, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Application notes: Optimal dilution of the CDw17 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: RT-97 + NR-4
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Neurofilament antibody cocktail is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament antibody (RT-97 + NR-4).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with Neurofilament antibody (RT-97 + NR-4).

Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody cocktail (clone RT-97 + NR-4); Red = isotype control, Blue = Neurofilament antibody cocktail.