Neurofilament Antibody Cocktail (Heavy + Light)

SKU: orb699506

Description

This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
• Clone No.: NF421 + NFL/736
• Immunogen: Recombinant human neurofilament protein was used as the immunogen for the Neurofilament antibody cocktail.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody cocktail (green) and Palloidin (red).

Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody cocktail (clone NF421 + NFL/736); Red=isotype control, Blue= Neurofilament antibody cocktail.

IHC staining of FFPE human cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P12036