Neurofilament Antibody Cocktail (Heavy + Light)

• Catalog Number: orb699506
• Category: Antibodies
• Description: This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NF421 + NFL/736
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
• Immunogen: Recombinant human neurofilament protein was used as the immunogen for the Neurofilament antibody cocktail.
• Dilution range: Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Neurofilament antibody cocktail is available for research use only.
• UniProt ID: P12036
• Storage: Store the Neurofilament antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.