NENF Antibody

SKU: orb1247216

Description

NENF Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NEDGSPNLDFKP
• Target: NENF
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NENF, neuron derived neurotrophic factor, CIR2, SCIRP10, SPUF, SCIRP10-related protein, Spinal cord injury related protein 10 , cell growth-inhibiting protein 47 neudesin secreted protein of unknown function, Neudesin,

Quick Database Links

Gene Symbol

NENF

UniProt

Q9UMX5

RefSeq (Protein):NP_037481.1