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Neudesin/NENF Antibody

SKU: orb19646

Description

Goat polyclonal antibody to NENF

Research Area

Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:16000, WB: 0.3 μg/ml
Predicted ReactivityBovine, Canine, Human, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetNeudesin / NENF
Protein SequenceNEDGSPNLDFKP
Molecular Weight18.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NENF antibody, anti neuron derived neurotrophic factor antibody, anti CIR2 antibody, anti SCIRP10 antibody, anti SPUF antibody, anti SCIRP10-related protein antibody, anti Spinal cord injury related protein 10 antibody, anti cell growth-inhibiting protein 47 neudesin secreted protein of unknown function antibody, anti Neudesin antibody
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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Neudesin/NENF Antibody (orb19646)

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Available Sizes

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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