Nestin antibody

• Catalog Number: orb345408
• Category: Antibodies
• Description: Nestin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1484-1500 of human Nestin protein.
• Concentration: 1.23 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:80,000, IHC: 5ug/ml, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and Mesocricetus auratus (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from mouse (93%) and rat (88%). Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: P48681
• NCBI: 38176300
• Storage: Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Nestin antibody, rabbit anti-NES antib
• Note: For research use only
• Application notes: Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC), and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of at 40X (left) and 20X (right) Tissue: Brain and Cerebellum (right) using Nestin antibody

Immunohistochemical staining of mouse kidney (Left) and human kidney (Right) tissue using Nestin antibody

Western blot analysis of human Nestin tissue (arrowhead) using Nestin antibody

Western blot analysis of mouse Nestin tissue (arrowhead) using Nestin antibody