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Nephrin antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | IHC-P: 1:100-600 (based on 0.5mg/ml), WB: 1:100-500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Nephrin. Please contact us for the exact immunogen sequence. The peptide is available as orb374936. |
| Target | Nephrin |
| Molecular Weight | 135 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−NPHS1 Antibody [orb1239664]
ELISA, IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
0.1 mg, 0.02 mgNephrin Rabbit Polyclonal Antibody [orb185637]
FC, IF, IHC-Fr, IHC-P, WB
Rabbit
Canine, Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
1 ml (Carrier free), 50 μl, 100 μl, 200 μl

Quality Guarantee
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IHC-P image of mouse spleen tissue using anti-Nephrin (2.5 ug/ml)

WB analysis of rat kidney (lane 1), rat spleen (lane 2), rat bladder (lane 3), rat spinal cord (lane 4) using Nephrin antibody (2.5 ug/ml)

IHC-P staining of mouse spleen tissue using Nephrin antibody (2.5 ug/ml)

IHC-P image of mouse spleen tissue using Nephrin antibody (2.5 ug/ml)

Western blot analysis of rat kidney (lane 1), rat spleen (lane 2), rat bladder (lane 3), rat spinal cord (lane 4) using Nephrin antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse kidney tissue using anti-Nephrin (2.5 ug/ml)

Immunohistochemical staining of rat bladder tissue using anti-Nephrin (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat spinal cord tissue using anti-Nephrin (2.5 ug/ml)

IHC-P staining of mouse kidney tissue using Nephrin antibody (2.5 ug/ml)
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Protocol Information
Nephrin antibody (orb322977)
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