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| Catalog Number | orb322977 |
|---|---|
| Category | Antibodies |
| Description | Nephrin antibody |
| Target | Nephrin |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Immunogen | KLH conjugated synthetic peptide derived from human Nephrin. Please contact us for the exact immunogen sequence. The peptide is available as orb374936. |
| UniProt ID | O60500, Q9R044, Q9QZS7 |
| MW | 135 kDa |
| Tested applications | ELISA, IHC-P, WB |
| Dilution range | IHC-P: 1:100-600 (based on 0.5mg/ml), WB: 1:100-500 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti CNF antibody, anti Nephrin antibody, anti Nep Read more... |
| Note | For research use only |
| Entrez | 4868 |
| NCBI | 004637, 004646, 11, 31 |

IHC-P image of mouse spleen tissue using anti-Nephrin (2.5 ug/ml)

WB analysis of rat kidney (lane 1), rat spleen (lane 2), rat bladder (lane 3), rat spinal cord (lane 4) using Nephrin antibody (2.5 ug/ml)

IHC-P staining of mouse spleen tissue using Nephrin antibody (2.5 ug/ml)

IHC-P image of mouse spleen tissue using Nephrin antibody (2.5 ug/ml)

Western blot analysis of rat kidney (lane 1), rat spleen (lane 2), rat bladder (lane 3), rat spinal cord (lane 4) using Nephrin antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse kidney tissue using anti-Nephrin (2.5 ug/ml)

Immunohistochemical staining of rat bladder tissue using anti-Nephrin (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat spinal cord tissue using anti-Nephrin (2.5 ug/ml)

IHC-P staining of mouse kidney tissue using Nephrin antibody (2.5 ug/ml)
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Rat | |
Human, Mouse | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Rabbit | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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