Nephrin (Y1210) antibody

• Catalog Number: orb1938998
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28264
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:2000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 134742 Da
• Target: This Nephrin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1191-1219 amino acids from human Nephrin.
• UniProt ID: O60500
• NCBI: NP_004637.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nephrin, Renal glomerulus-specific cell adhesion r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Nephrin (Y1210) antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A-498 whole cell lysate. Lane 3: Caki-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 135 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Nephrin (Y1210) antibody western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).

Nephrin (Y1210) antibody western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).

Nephrin Antibody (Y1210) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.