NEK2 Antibody

• Catalog Number: orb1538570
• Category: Antibodies
• Description: NEK2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2F6
• Tested applications: ELISA, IHC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG2a,k
• Immunogen: NEK2 (AAH43502, 331 aa ~ 445 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml), IP, WB
• Conjugation: Unconjugated
• Target: NEK2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Alternative names: NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-l
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using transfected cell lysates and the recombinant protein used as the immunogen.
• Expiration Date: 12 months from date of receipt.

Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.

Detection limit for recombinant GST tagged NEK2 is approximately 0.3 ng/ml as a capture antibody.