NEK2 Antibody (Center)

• Catalog Number: orb1788313
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 396-426 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 51763 Da
• Target: This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the Central region of human NEK2.
• UniProt ID: P51955
• NCBI: NP_001191112.1, NP_001191111.1, NP_002488.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NEK2; NEK2A; NLK1; Serine/threonine-protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded M. brain section using NEK2 Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. brain section using NEK2 Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. brain section using NEK2 Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, K562, MCF-7, mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Fluorescent image of U251 cells stained with hNEK2-C410. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).