NEIL1 Antibody

• Catalog Number: orb1247220
• Category: Antibodies
• Description: NEIL1 Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DIPSLEPEGTSAS
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NEIL1
• UniProt ID: Q96FI4
• NCBI: NP_078884.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247220 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.