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Description
Research Area
Epigenetics & Chromatin
Images & Validation
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| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 60kDa band observed in lysates of cell lines Daudi and U937, and in nuclear cell lysates of HEK293 (calculated MW of 53.0kDa according to NP_001243481.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration: 5-10µg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U937 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Porcine |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | NEIL1 / NEH1 |
| Protein Sequence | DIPSLEPEGTSAS |
| Molecular Weight | 43.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti NEIL1 antibody, anti NEH1 antibody, anti nei endonuclease VIII-like 1 (E. coli) antibody, anti NEI1 antibody, anti FLJ22402 antibody, anti endonuclease VIII antibody, anti FPG1 antibody, anti hFPG1 antibody, anti nei endonuclease VIII-like 1 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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