NEFM/NF-M Antibody

SKU: orb1535281

Description

NEFM/NF-M Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.1 µg/ml)
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1 ug/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.
• Target: NEFM / NF-M
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFM, 160 kDa neurofilament protein, NEF3, NF-M, NFM, Neurofilament 3

NEFM antibody (0.03 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NEFM / NF-M