NEFH Antibody

• Catalog Number: orb1255836
• Category: Antibodies
• Description: NEFH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 240kDa
• Target: NEFH
• UniProt ID: P12036
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse brain, using NEFH antibody (orb1255836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of mouse brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.