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NEFH Antibody
SKU: orb1255836
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2). |
| Target | NEFH |
| Molecular Weight | Observed: 240kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
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Western blot analysis of extracts of mouse brain, using NEFH antibody (orb1255836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
NEFH
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
NEFH Antibody (orb1255836)
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