NDUFA7 Antibody, KO Validated

• Catalog Number: orb1274412
• Category: Antibodies
• Description: NDUFA7 Antibody, KO Validated
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 13kDa
• Target: NDUFA7
• UniProt ID: O95182
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: B14.5a, CI-B14.5a, complex I B14.5a subunit, NADH
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (orb1274412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (orb1274412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human liver damage using NDUFA7 antibody (orb1274412) at dilution of 1:100 (40x lens).