NDUFA7 Antibody

SKU: orb43519

Description

Goat polyclonal to NDUFA7.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 4-8 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  WB: Approx 15kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 12.6kDa according to NP_004992.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.. IHC: In paraffin embedded Human Heart shows mitochondrial staining in cardial fibres. Recommended concentration: 4-8µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NDUFA7
• Protein Sequence: PKLPVGPSHKLSNN
• Molecular Weight: 12.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti B14.5a antibody, CIB14.5a antibody, complex I B14.5a subunit antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa antibody, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 antibody, NADHubiquinone oxidoreductase subunit B14.5a antibody, NDUFA7 antibody

Western blot analysis of staining of Pig Heart lysate using NDUFA7 antibody.

Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Heart lysates using NDUFA7 antibody.

Immunohistochemical staining of Human Heart using NDUFA7 antibody

Quick Database Links

Gene Symbol

NDUFA7

NCBI Gene ID

4701