NDRG2 Antibody

• Catalog Number: orb1249248
• Category: Antibodies
• Description: NDRG2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: KEAELAARILLDQGQ-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NDRG2
• UniProt ID: Q9UN36
• NCBI: NP_963293.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NDRG2, NDRG family member 2, DKFZp781G1938, FLJ255
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249248 (0.1 ug/ml) staining of Human Temporal Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249248 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249248 (3.75 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.