NDRG1 Antibody

• Catalog Number: orb1247224
• Category: Antibodies
• Description: NDRG1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: GNSAGPKSMEVSC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2ug/ml. Paraffin embedded Human Prostate and Colon. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NDRG1
• UniProt ID: Q92597
• NCBI: NP_006087.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NDRG1, N-myc downstream regulated gene 1, CAP43,,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2ug/ml. Paraffin embedded Human Prostate and Colon. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247224 (0.1 ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247224 (0.5 ug/ml) staining of paraffin embedded Human hepatocelluar carcinoma.

orb1247224 (2.5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247224 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.