Goat anti-NDRG1 Antibody

• Catalog Number: orb18830
• Category: Antibodies
• Description: Goat polyclonal antibody to NDRG1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Concentration: 0.5-2 μg/ml
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 42.8
• Target: NDRG1
• Entrez: 10397
• Protein Sequence: GNSAGPKSMEVSC
• RRID: AB_10755725
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NDRG1 antibody, anti N-myc downstream regulat
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of Human Brain, Kidney and Testes (calculated MW of 42.8kDa according to NP_006087.2). Recommended concentration: 0.1-0.3 μg/ml. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb18830 staining (0.1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of Human Kidney (Lane 1), Cerebellum (Lane 2) and Testis (Lane 3) lysate using NDRG1 antibody

Immunohistochemical staining of Human hepatocelluar carcinoma using NDRG1 antibody

Immunohistochemical staining of Human Prostate using NDRG1 antibody

Immunohistochemical staining of Human Colon using NDRG1 antibody