NCLN Antibody

• Catalog Number: orb1239390
• Category: Antibodies
• Description: NCLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN.The immunogen is located within amino acids 390 - 440 of NCLN.
• Concentration: 1 mg/mL
• Dilution range: NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCLN
• UniProt ID: Q969V3
• NCBI: NP_064555
• Storage: NCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NCLN Antibody: NET59, Nicalin, Nicastrin-like prot
• Note: For research use only
• Application notes: NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 µg/mL.

Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 µg/mL.