Rabbit anti-NCLN Antibody

• Catalog Number: orb1520737
• Category: Antibodies
• Description: NCLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 375 and 425
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NCLN
• UniProt ID: Q969V3
• NCBI: Q969V3.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: NET59; nicalin; nicalin homolog; nicastrin-like pr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse NCLN by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NCLN by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for IP at 1 µg per reaction.