NCK1 Antibody

SKU: orb97467

Description

Mouse monoclonal antibody to NCK1

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 5B7C2
• Immunogen: Purified recombinant fragment of human NCK1 expressed in E. Coli.
• Target: NCK1
• Molecular Weight: 47kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

NCK; nck-1; NCKalpha

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NCK1 antibody

Western blot analysis of human (AA: 203-371) recombinant protein using NCK1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded rectum cancer tissues using NCK1 antibody

Flow cytometric analysis of Jurkat cells using NCK1 antibody

Western blot analysis of Jurkat (Lane1), HeLa (Lane2), HEK293 (Lane3), A431 (Lane4), K562 (Lane5), and COS7 (Lane6) cell lysate using NCK1 antibody

Quick Database Links

Gene Symbol

NCK1

UniProt

P16333

NCBI Gene ID

4690