NCK1 Antibody

• Catalog Number: orb97467
• Category: Antibodies
• Description: Mouse monoclonal antibody to NCK1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5B7C2
• Tested applications: FC, IHC, WB
• Reactivity: Human, Monkey
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human NCK1 expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 47kDa
• Target: NCK1
• Entrez: 4690
• UniProt ID: P16333
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: NCK; nck-1; NCKalpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NCK1 antibody

Western blot analysis of human (AA: 203-371) recombinant protein using NCK1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded rectum cancer tissues using NCK1 antibody

Flow cytometric analysis of Jurkat cells using NCK1 antibody

Western blot analysis of Jurkat (Lane1), HeLa (Lane2), HEK293 (Lane3), A431 (Lane4), K562 (Lane5), and COS7 (Lane6) cell lysate using NCK1 antibody