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Catalog Number | orb1936453 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB33697 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 42864 Da |
Target | This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK1. |
UniProt ID | P16333 |
NCBI | NP_006144.1, NP_001177725.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Cytoplasmic protein NCK1, NCK adaptor protein 1, N Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
NCK1 Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the NCK1 antibody detected the NCK1 protein (arrow).
NCK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NCK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.