NCAM1 Antibody

SKU: orb1256097

Description

NCAM1 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
• Target: NCAM1
• Molecular Weight: Observed: 160kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

neural cell adhesion molecule, NCAM, antigen recognized by monoclonal 5.1H11, NCAM-1, N-CAM-1, MSK39, CD56, neural cell adhesion molecule 1, NCAM1

Western blot analysis of extracts of various cell lines, using NCAM1/CD56 antibody (orb1256097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human stomach using NCAM1/CD56 antibody (orb1256097) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using NCAM1/CD56 antibody (orb1256097) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NCAM1

UniProt

P13591