NCAM / CD56 Antibody

• Catalog Number: orb385892
• Category: Antibodies
• Description: CD56/NCAM is a cell adhesion protein which is a member of the immunoglobulin superfamily. It is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. CD56 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA56
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the CD56 antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD56 antibody is available for research use only.
• UniProt ID: P13591
• Storage: Store the CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the CD56 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human lung carcinoma using CD56 antibody

Immunohistochemical staining of Human brain tumor using CD56 antibody

Western blot analysis of HCT116 cell lysate using CD56 antibody