NCAM / CD56 Antibody

• Catalog Number: orb385892
• Category: Antibodies
• Description: CD56/NCAM is a cell adhesion protein which is a member of the immunoglobulin superfamily. It is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. CD56 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the CD56 antibody.
• UniProt ID: P13591
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Application notes: Titering of the CD56 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: CDLA56
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This CD56 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human lung carcinoma with CD56 antibody.

IHC testing of FFPE human brain tumor with CD56 antibody.

Western blot testing of HCT116 cell lysate and CD56 antibody. Observed molecular weights: 120, 140 and 180 kDa.