Napabucasin

SKU: orb1304413

Description

Napabucasin (BBI608) is an orally bioavailable small molecule inhibitor targeting STAT3 and cancer stemness pathways. It has been investigated in preclinical in vitro and in vivo studies for its potential in oncology research, particularly against various solid tumors.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 83280-65-3
• MW: 240.21
• Purity: 99.85% (May vary between batches)
• Formula: C₁₄H₈O₄
• SMILES: CC(=O)c1cc2c(o1)C(=O)c1ccccc1C2=O
• Target: STAT
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);acetonitrile:0.8 mg/mL (3.33 mM);DMSO:6.88 mg/mL (28.64 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  In mice bearing PaCa-2 xenografts, Napabucasin (20 mg/kg, i.p.) significantly inhibits tumor growth, relapse and metastasis.
• In Vitro:
  Napabucasin downregulates stemness gene expression driven by Stat3 and cancer stemness properties, and effectively inhibits self-renewal of stemness-high cancer cells with IC50 ranged from 0.291~1.19 μM, on inhibition on normal stem cells.
• Cell Research:
  For cancer stem cells, spheres are dissociated and plated under cancer stem cell culture conditions on coated 96-well plates. After 72 h of culture, wells are dosed with the indicated compounds. Seventy-two hours or 24 h after dosing, CellTiter-Glo 2.0 is added to each well, and the luminescence is measured as described by the manufacturer. IC50 values are calculated by fitting a four parameter dose–response curve to normalized data using GraphPad Prism software. For bulk cells, cells are plated at 5,000 cells per well on 96-well plates. Twenty-four hours after plating, cells are treated with the indicated compounds. Viability is determined at 72 h as described above.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BBI 608, BBI608, BBI-608, Napabucasin, inhibit, Inhibitor, STAT3, STAT

Compound Identifiers

PubChem CID

10331844