N6AMT1 Antibody

SKU: orb1256532

Description

N6AMT1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3).
• Target: N6AMT1
• Molecular Weight: Observed: 23kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

C21orf127, HEMK2, MTQ2, N6AMT, PRED28, m.HsaHemK2P, methyltransferase N6AMT1, HemK methyltransferase family member 2, N-6 adenine-specific DNA methyltransferase 1 (putative), N6-DNA-methyltransferase, methylarsonite methyltransferase N6AMT1, protein N(5)-glutamine methyltransferase

Western blot analysis of extracts of various cell lines, using N6AMT1 antibody (orb1256532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using N6AMT1 Antibody (orb1256532) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using N6AMT1 Antibody (orb1256532) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using N6AMT1 antibody (orb1256532). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

N6AMT1

UniProt

Q9Y5N5