Myosin IH Antibody

SKU: orb1248384

Description

Myosin IH Antibody

Research Area

Cancer Biology, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3µg/mL. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EDQVYKDKNGQLR
• Target: Myosin IH
• Molecular Weight: Approx 110kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventional myosin-Ih

orb1248384 (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248384 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Myosin IH

UniProt

Q8N1T3

RefSeq (Protein):NP_001158045.1