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Catalog Number | orb1248384 |
---|---|
Category | Antibodies |
Description | Myosin IH Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Porcine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-EDQVYKDKNGQLR |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 110kDa |
Target | Myosin IH |
UniProt ID | Q8N1T3 |
NCBI | NP_001158045.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventi Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248384 (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248384 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ICC, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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