Myosin IH Antibody

SKU: orb131704

Description

Goat polyclonal to Myo1H

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Myosin IH
• Protein Sequence: EDQVYKDKNGQLR
• Molecular Weight: 118
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MYO1H; myosin IH; myosin-1H; myosin-Ih; unconventional myosin-Ih

Western blot analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using Myo1H antibody

Immunohistochemical staining of Human Kidney using Myo1H antibody

Quick Database Links

Gene Symbol

Myosin IH

NCBI Gene ID

283446