Myogenin Antibody [SPM144]

• Catalog Number: orb1251912
• Category: Antibodies
• Description: Myogenin Antibody [SPM144]
• Target: MYOG
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Feline, Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Human recombinant protein was used as the immunogen for this anti-Myogenin antibody.
• UniProt ID: P15173
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Application notes: ELISA: order the BSA/azide free formatFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunoprecipitation: 0.5-1 ug/500ug protein lysateImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-Myogenin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM144
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Myogenin, Class C basic helix-loop-helix protein 3
• Note: For research use only

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with anti-Myogenin antibody (SPM144)