Myogenin Rabbit Polyclonal Antibody

SKU: orb6492

Description

Myogenin Rabbit Polyclonal Antibody

Research Area

Developmental Biology, Epigenetics and Nuclear Signaling, Myogenesis

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MYOG/Myogenin (65-180/224aa)
• Target: MYOG
• Molecular Weight: 25 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MYOG_HUMAN; MYOG; BHLHC3; MYF4; Class C basic helix-loop-helix protein 3(bHLHc3); Myogenic factor 4(Myf-4); BHLHC3; MYF4; MYF 4;

Immunohistochemical analysis of formalin-fixed and paraffin embedded rat heart tissue (dilution at:1:200) using Myogenin antibody

Western blot analysis of rat heart lysates (Lane1), mouse skeletal muscle lysates (Lane2) dilution at:1:200 using Myogenin antibody

Quick Database Links

Gene Symbol

MYOG

UniProt

P15173

References

Ma, Manting et al. lncRNA-Six1 Is a Target of miR-1611 that Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis Cells, 7, E243 (2018)

PubMed: 30518151

Applications

WB

Reactivity

Gallus

Cai, Bolin et al. MiR-16-5p targets SESN1 to regulate the p53 signaling pathway, affecting myoblast proliferation and apoptosis, and is involved in myoblast differentiation Cell Death Dis, 9, 367 (2018)

PubMed: 29511169

Applications

WB

Reactivity

Gallus

Bolin Cai 1 2, Manting Ma 1 2, Rongshuai Yuan 1 2, Zhen Zhou 1 2, Jing Zhang 3, Shaofen Kong 1 2, Duo Lin 1 2, Ling Lian 4, Juan Li 5, Xiquan Zhang 1 2, Qinghua Nie MYH1G-AS is a chromatin-associated lncRNA that regulates skeletal muscle development in chicken Cell Mol Biol Lett ., 29, 9 (2024)

PubMed: 38177995

Applications

WB

Reactivity

Gallus

L Wei, Y Li, H Tan, Y Peng, Q Liu, T Zheng, F Li, Z Xu OTUB1 regulates ferroptosis to inhibit myoblast differentiation into myotubes by deubiquitinating P62 Scientific Reports, (2024)

PubMed:

Applications

WB

Reactivity

Mouse

Li, Zhenhui et al. LncIRS1 controls muscle atrophy via sponging miR-15 family to activate IGF1-PI3K/AKT pathway J Cachexia Sarcopenia Muscle, (2019)

PubMed: 30701698

Applications

WB

Reactivity

Gallus