MYOG Antibody

SKU: orb1252377

Description

MYOG Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Canine, Feline, Human, Mouse, Porcine, Rat
• Application Notes:
  ELISA: order BSA-free formatFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mLImmunoprecipitation: 0.5-1 ug/500ug protein lysateImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: F5D
• Immunogen: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
• Target: MYOG
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myogenin, Class C basic helix-loop-helix protein 3, bHLHc3, Myogenic factor 4, Myf-4, MYOG, BHLHC3, MYF4

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D).

Quick Database Links

Gene Symbol

MYOG

UniProt

P15173