MYLIP Antibody (Center)

SKU: orb1930568

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MYLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the Central region of human MYLIP. Antigen Region: 111-139 aa.
• Target: MYLIP
• Molecular Weight: 49910 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase MYLIP, 632-, Inducible degrader of the LDL-receptor, Idol, Myosin regulatory light chain interacting protein, MIR, MYLIP, BZF1, IDOL

MYLIP Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MYLIP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

MYLIP Antibody (Center T126) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).

Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Anti-MYLIP Antibody (Center) at 1:2000 dilution + Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MYLIP Antibody (Center) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

MYLIP

UniProt

Q8WY64

RefSeq (Protein):NP_037394.2