IDOL Rabbit Polyclonal Antibody

SKU: orb256703

Description

The IDOL Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDOL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200
• Reactivity: Bovine, Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDOL. The exact sequence is proprietary.
• Target: MYLIP
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BZF1; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR

Western blot analysis of IDOL expression in Hela (A), COS7 (B), MCF7 (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)

Immunohistochemical analysis of IDOL staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

MYLIP

UniProt

Q8WY64, Q8BM54

NCBI Gene ID

218203, 29116