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MYL9 Antibody

SKU: orb1257974

Description

MYL9 Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MYL9 (NP_006088.2).
TargetMYL9
Molecular WeightObserved: 20kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC2, Myosin RLC, MRLC1, MYRL2

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MYL9 Antibody

Western blot analysis of extracts of various cell lines, using MYL9 antibody (orb1257974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

MYL9 Antibody

Immunofluorescence analysis of Rat intestine using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.

MYL9 Antibody

Immunofluorescence analysis of Human colon carcinoma using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.

MYL9 Antibody

Immunofluorescence analysis of Mouse intestine using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

MYL9 Antibody (orb1257974)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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