MYL9 Antibody

• Catalog Number: orb1257974
• Category: Antibodies
• Description: MYL9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MYL9 (NP_006088.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 20kDa
• Target: MYL9
• UniProt ID: P24844
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MYL9 antibody (orb1257974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of Rat intestine using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Human colon carcinoma using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Mouse intestine using MYL9 antibody (orb1257974) at dilution of 1:100. Blue: DAPI for nuclear staining.