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| Catalog Number | orb768755 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to MRLC2 (phospho-Ser18) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18. AA range:3-52 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 18 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-MYL9 antibody, anti-MLC2 antibody, anti-MRLC1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody.
Immunofluorescence analysis of HUVEC cells, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from LOVO cells treated with H2O2 100uM 30', using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The lane on the right is blocked with the phospho peptide.
