Myeloid Cell Marker Antibody

SKU: orb2638085

Description

This antibody recognizes a 183kDa protein with DNA-binding characteristics, which is identified as a myeloid cell specific antigen. Clone BM-1 antibody reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. This type of marker is useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. This mAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of the marker protein is not clear, although it has been proposed that it may play a role in cell differentiation.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myeloid cell marker antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: BM-1
• Immunogen: Human peripheral blood mononuclear cells were used as the immunogen for this Myeloid cell marker antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin/paraffin human tonsil stained with Myeloid cell marker antibody (BM-1).

SDS-PAGE Analysis of Purified, BSA-Free Myeloid Cell Marker Antibody (clone BM-1). Confirmation of Integrity and Purity of the Antibody.