MYB Antibody (Center)

• Catalog Number: orb1788262
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This MYB antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from the Central region of human MYB.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,κ
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Immunogen: 1-185 aa
• UniProt ID: P10242
• MW: 72341 Da
• Tested applications: WB
• Dilution range: WB: 1:2000, WB: 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcriptional activator Myb, Proto-oncogene c-My
• Note: For research use only

Western blot analysis of lysates from CEM, Jurkat cell line (from left to right), using MYB Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

All lanes: Anti-MYB Antibody (Center) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.