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Anti-A-MYB Antibody

Catalog Number: orb216163

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb216163
CategoryAntibodies
DescriptionRabbit polyclonal antibody to A-MYB
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Porcine, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human A-MYB. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMYBL1
Entrez17864, 4603
UniProt IDP51960, P10243
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti AMYB antibody, anti Myb-related protein A ant
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-A-MYB Antibody

Western blot analysis of A-MYB expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 90 kD)

Anti-A-MYB Antibody

Immunofluorescent analysis of A-MYB staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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    100 μl
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