Mutarotase Antibody Biotin Conjugated

SKU: orb344966

Description

Mutarotase antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  Anti-Mutarotase antibody has been tested in WB and assayed against 1.0 ug of Mutarotase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mutarotase [Porcine Kidney]
• Purity: Anti-Mutarotase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney]. Cross reactivity against Mutarotase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mutarotase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody

ELISA Results of Rabbit Anti-Mutarotase Antibody Biotin Conjugated tested against purified Mutarotase. Each well was coated in duplicate with 1.0 µg of Mutarotase. The working dilution is 4100. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer and TMB substrate (p/n orb348651).

Western Blot of Rabbit Anti-Mutarotase Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Mutarotase [0.05 µg]. Primary Antibody: Anti-Mutarotase Antibody Biotin at 1:1000 overnight at 2-8°C. Secondary Antibody: HRP Streptavidin (p/n orb348767) at 1:40000 for 30 min at RT. Blocking Buffer: BlockOut Buffer (p/n orb348644) for 30 min at RT. Predicted MW: ~38kDa.

Quick Database Links

UniProt

Q9GKX6

RefSeq:Q9GKX6.1