ACTA2 Antibody

SKU: orb1251988

Description

ACTA2 Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Reactivity: Human, Rat
• Application Notes:
  Western blot: 0.5-1 µg/mLImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Muscle Actin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: PMAC1-1
• Immunogen: An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.
• Target: ACTA2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin, ACTVS, GIG46

IHC testing of FFPE human leiomyosarcoma with Muscle Actin antibody

IHC testing of FFPE human rhabdomyosarcoma with Muscle Actin antibody

IHC testing of FFPE rat skeleton muscle with Muscle Actin antibody

IHC testing of FFPE rat heart with Muscle Actin antibody

Western blot testing of human skeletal muslce with Muscle Actin antibody. Predicted molecular weight ~42 kDa.

Quick Database Links

Gene Symbol

ACTA2

UniProt

P62736