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MUC5AC Antibody

Catalog Number: orb749692

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749692
CategoryAntibodies
DescriptionMucin 5AC glycoprotein (MUC5AC) is a HMW glycoprotein belonging to the superfamily of mucins. Mucins are produced by epithelial cells and can be divided into two families; secretory mucins and membrane bound mucins. MUC5AC is a mucus-forming, secreted mucin that is found in normal gastric and tracheo-bronchial mucosa, but absent from normal colon. MUC5AC expression is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenA synthetic peptide of human MUC5AC tandem repeat was used as the immunogen for the MUC5AC antibody.
UniProt IDP98088
Tested applicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesOptimal dilution of the MUC5AC antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberCLH2
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the MUC5°C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis MUC5AC antibody is available for research use only.
NoteFor research use only
MUC5AC Antibody

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

MUC5AC Antibody

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

MUC5AC Antibody

SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone CLH2). Confirmation of Integrity and Purity of the Antibody.

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