MUC5AC (Mucin 5AC / Gastric Mucin) Antibody

SKU: orb1410089

Description

Mouse monoclonal antibody to MUC5AC (Mucin 5AC / Gastric Mucin)

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution range: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Stomach

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 45M1
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Target: MUC5AC
• Source/Expression System: HEK293 cells
• Molecular Weight: 400-600 kDa
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% azide and 0.05% rAlbumin. Contact us if you require this Ab in a different format.
• Concentration: Purified Ab conjugated to Biotin; supplied with BSA and Azide at 200ug/ml
• Disclaimer: For research use only

Alternative Names

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Biotinylated MUC5AC Mouse Monoclonal Antibody (45M1).

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Mouse Monoclonal Antibody (45M1).

Formalin-fixed, paraffin-embedded human Stomach stained with MUC5AC Mouse Monoclonal Antibody (45M1).

Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Mouse Monoclonal Antibody (45M1).

SDS-PAGE Analysis Purified MUC5AC Mouse Monoclonal Antibody (45M1). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

MUC5AC

UniProt

P98088

NCBI Gene ID

4586